peroxiredoxin 2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

peroxiredoxin 2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H6C4

Cat no : 60202-1-PBS

Synonyms

NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA

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Validation Data Gallery

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  WB analysis using 60202-1-Ig (same clone as 60202-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60202-1-Ig (same clone as 60202-1-PBS)

  WB result of peroxiredoxin 2 antibody (60202-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60202-1-Ig (same clone as 60202-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60202-1-Ig (same clone as 60202-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60202-1-Ig (same clone as 60202-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60202-1-PBS targets peroxiredoxin 2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: peroxiredoxin 2 fusion protein Ag0835
• Full Name: peroxiredoxin 2
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC003022
• Gene Symbol: PRDX2
• Gene ID (NCBI): 7001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)