peroxiredoxin 2 Monoclonal antibody

• Featured Product
• KD/KO Validated

peroxiredoxin 2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

3H6C4

Cat no : 60202-1-Ig

Synonyms

PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B

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Validation Data Gallery

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  WB analysis using 60202-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60202-1-Ig

  WB result of peroxiredoxin 2 antibody (60202-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells.

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  WB analysis of MCF-7 using 60202-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 60202-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 60202-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60202-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 60202-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using peroxiredoxin 2 antibody (60202-1-Ig, Clone: 3H6C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, human testis tissue, fetal human brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
• Positive IHC detected in: human testis tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

60202-1-Ig targets peroxiredoxin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: peroxiredoxin 2 fusion protein Ag0835
• Full Name: peroxiredoxin 2
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC003022
• Gene Symbol: peroxiredoxin 2
• Gene ID (NCBI): 7001
• RRID: AB_10918978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

Publications

Species	Application	Title
human	WB,IHC,IF	J Exp Clin Cancer Res; Compound kushen injection suppresses human acute myeloid leukaemia by regulating the Prdxs/ROS/Trx1 signalling pathway.; Authors - Yanxia Jin
human	WB	Cancer Manag Res; siPRDX2-elevated DNM3 inhibits the proliferation and metastasis of colon cancer cells via AKT signaling pathway.; Authors - Yini Ma ; KD Validated
human	WB	Oncol Lett; PRDX2 protects hepatocellular carcinoma SMMC-7721 cells from oxidative stress.; Authors - Silei Zhou
human	IF	Cell Death Dis; PRDX2 promotes the proliferation of colorectal cancer cells by increasing the ubiquitinated degradation of p53.; Authors - Wuyi Wang ; KD Validated
human	WB	Curr Issues Mol Biol; Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients; Authors - Fadime Aydın Köse