ChromoTek p53 C-term-Trap Agarose, kit

p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.

Specificity

p53

Reactivity

Human, Mouse

Applications

IP, CoIP

Conjugate

Agarose

Type

Nanobody

Publications

1

Cat no : pta2k

Synonyms

p53 C-term-Trap, p53 Trap, p53-Trap,Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

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Validation Data Gallery

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  p53-C-term-Trap Agarose for Immunoprecipitation of p53.I: Input, FT: Flow-through, B: Bound.

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Product Information

p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.

• Description: The p53-C-term-Trap Agarose Kit contains p53-C-term-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of p53.

  • Immunoprecipitation of human and mice p53 isoforms alpha with p53-C-term-Trap

  • Pull-down of the human p53 proteome with complementary p53-N-term- and p53-C-term-Traps, e.g. p53 isoforms and variants

  • No heavy & light antibody chains in your downstream application

  • Short incubation time (60 min at +4°C)

  • Strong binding even under harsh washing conditions

• Applications: IP, CoIP
• Specificity/Target: p53 (isoform alpha); epitope 302-393 aa
• Tested Reactivity: Human, Mouse
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Compatibility with mass spectrometry: The p53-C-term-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631379
• Storage Buffer: 20% ethanol
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Kit components

Component	Description
p53 C-term-Trap Agarose	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution

Other Formats

p53 C term-Trap Agarose

Publications

Application	Title
IP	Am J Respir Crit Care Med; Long Noncoding RNA TYKRIL Plays a Role in Pulmonary Hypertension via the p53-mediated Regulation of PDGFRβ.; Authors - Christoph M Zehendner