p300 Polyclonal antibody, PBS Only

p300 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20695-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 20695-1-AP (same clone as 20695-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20695-1-AP (same clone as 20695-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20695-1-AP (same clone as 20695-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 20695-1-AP (same clone as 20695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 20695-1-AP (same clone as 20695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 20695-1-AP (same clone as 20695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

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It is recommended to use NETN lysis buffer for sample preparation during WB detection.

Product Information

20695-1-PBS targets p300 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: E1A binding protein p300
• Calculated Molecular Weight: 264 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: NM_001429
• Gene Symbol: EP300
• Gene ID (NCBI): 2033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).