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Mouse p21 Polyclonal antibody, PBS Only

p21 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28248-1-PBS

Synonyms

Cdkn1a, p21WAF, p21Cip1, P21 protein, Cdkn1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p21 Polyclonal antibody (28248-1-AP)

    WB analysis using 28248-1-AP (same clone as 28248-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using p21 Polyclonal antibody (28248-1-AP)

    WB analysis using 28248-1-AP (same clone as 28248-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using p21 Polyclonal antibody (28248-1-AP)

    IF Staining of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 28248-1-AP (p21 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red). This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using p21 Polyclonal antibody (28248-1-AP)

    IF Staining of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using p21 antibody (28248-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

Product Information

28248-1-PBS targets p21 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p21 fusion protein Ag28394
Full Name cyclin-dependent kinase inhibitor 1A (P21)
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberNM_001111099
Gene Symbol p21
Gene ID (NCBI) 12575
RRIDAB_2881097
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.