CoraLite® Plus 488-conjugated golgin 97 Polyclonal antibody

golgin 97 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12640

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1

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Validation Data Gallery

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  IF Staining of HeLa using CL488-12640

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 golgin 97 antibody (CL488-12640) at dilution of 1:400, CL594-phalloidin (red).

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  FC experiment of HeLa using CL488-12640

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human golgin 97 (CL488-12640) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12640 targets golgin 97 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: golgin 97 fusion protein Ag3335
• Full Name: golgi autoantigen, golgin subfamily a, 1
• Calculated Molecular Weight: 767 aa, 88 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC032853
• Gene Symbol: golgin 97
• Gene ID (NCBI): 2800
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa. IF result shows strong Gogli staining.