Recombinant Antibodies for Flow Cytometry
Proteintech offers a wide selection of recombinant primary antibodies validated in and optimized for flow cytometry from CD markers to anti-cytokine antibodies and intracellular targets.
Gold Standard clones made recombinantly
Antibodies with the epitope and binding affinity of top-cited clones for flow cytometry produced recombinantly with Rabbit and Mouse host isotypes.
New recombinant antibody clones optimized for flow cytometry
Surface marker and cytokine antibodies generated de novo, validated and formulated for optimal performance in flow cytometry.
FcZero-rAb for Flow Cytometry
Flow antibodies with a mutated Fc receptor binding region that eliminates non-specific background from Fc receptor binding.
Nanobodies for flow cytometry
Fluorescently conjugated nanobodies (VHH) for immuno-oncology targets with increased sensitivity and decreased background in flow cytometry.
New
FcZero-rAb™ are recombinantly expressed antibodies for flow cytometry that eliminates background signal due to Fc Receptor binding – without the need for Fc Receptor block.
Why use recombinant antibodies?
Consistent performance
Rigorous validation
Available with rabbit and mouse host isotypes
Continuous supply
BSA and azide-free formulation available
Gold Standard Clones - Reimagined
The antigen-binding region of top-cited clones for flow cytometry have been recombined with Rabbit IgG and Mouse IgG1 backbones for the consistency of recombinant antibodies and the performance of gold-standard clones you know and trust.
1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD8 Rabbit Recombinant Antibody (65510-1-RR, Clone:UCHT4) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.1 ug Anti-Human CD8 Mouse Monoclonal Antibody (right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
De novo recombinant clones optimized for flow cytometry
Proteintech recombinant antibody clones are carefully selected after multiple rounds of validation in applications such as ELISA, flow cytometry, WB, and IHC. Recombinant antibodies for flow cytometry are validated with both fixed and unfixed staining (if applicable) to find the clone with the best signal intensity and lowest background.
Proteintech Recombinant Antibodies for Flow Cytometry have similar or superior performance compared to gold-standard clones.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR, Clone:241347E1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed.
Related products
Recombinant Antibodies
Genetically defined monoclonals, recombinantly produced. Unrivalled reproducibility. Good for long-term, high-level projects.
All Antibodies for Flow Cytometry
Primary antibodies conjugated to a variety of fluorescent dyes for use in flow cytometry.
Spectra Viewer
Plan and design your multicolor panel experiments using our Spectra Viewer to view excitation and emission of different fluorophores.
Supporting Reagents & Kits
Reagents to support your flow cytometry experiments: fix & perm buffers, isotype control antibodies, secondary antibodies, viability dyes, and more.