1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD45 (65082-1-Ig, Clone: 2D1) and Cy3–conjugated Affinipure Goat Anti-Mouse IgG(H+L) (SA00009-1) at dilution 1:50 (green), and 0.5 ug Control Antibody. Cells were not fixed.