Dynamitin (1-401 AA) Monoclonal antibody

Dynamitin (1-401 AA) Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2B9A5

Cat no : 60257-1-Ig

Synonyms

DCTN2, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, Dynamitin

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Validation Data Gallery

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  WB analysis of human brain using 60257-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60257-1-Ig (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 60257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60257-1-Ig (Dynamitin antibody at dilution of 1:50.

• 

  IHC staining of human gliomas using 60257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60257-1-Ig (Dynamitin antibody at dilution of 1:50.

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 60257-1-Ig is Dynamitin (1-401 AA) Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: Dynamitin (1-401 AA) fusion protein Ag1902
• Full Name: dynactin 2 (p50)
• Calculated Molecular Weight: 401 aa, 44 kDa
• Observed Molecular Weight: 52+150 kDa
• GenBank Accession Number: BC014083
• Gene Symbol: Dynamitin
• Gene ID (NCBI): 10540
• RRID: AB_2881378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is is against the N terminal 86aa of full length p50 dynamitin.

Publications

Species	Application	Title
	IF	Dev Biol; Knockdown of Dynamitin in testes significantly decreased male fertility in Drosophila melanogaster.; Authors - Chun-Hong Wu