Dynamitin (1-401 AA) Monoclonal antibody, PBS Only

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  WB analysis of human brain using 60257-1-Ig (same clone as 60257-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 60257-1-Ig (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60257-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60257-1-Ig (same clone as 60257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60257-1-Ig (Dynamitin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60257-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60257-1-Ig (same clone as 60257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60257-1-Ig (Dynamitin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60257-1-PBS in a different storage buffer formulation.

Dynamitin (1-401 AA) Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 60257-1-PBS

Clone No.2B9A5

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, Dynamitin, Dynamitin (1-401 AA), p50 dynamitin, RBP50

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Product Information

60257-1-PBS targets Dynamitin (1-401 AA) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: Dynamitin (1-401 AA) fusion protein Ag1902
• Full Name: dynactin 2 (p50)
• Calculated Molecular Weight: 401 aa, 44 kDa
• Observed Molecular Weight: 52+150 kDa
• GenBank Accession Number: BC014083
• Gene Symbol: Dynamitin
• Gene ID (NCBI): 10540
• RRID: AB_2881378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13561
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is is against the N terminal 86aa of full length p50 dynamitin.