Nanobody-based Reagents
Nanobodies are the smallest known antibodies (~12-15 kDa vs ~150 kDa of conventional IgG antibodies). They are the antigen binding domains or variable heavy domain of heavy chain antibodies (VHHs) from alpacas, llamas, or camels.
ChromoTek Nanobody-based reagents are thoroughly validated, recombinantly expressed, and ready to use. Due to their size and single chain characteristic, they provide a higher level of performance than conventional IgG antibodies in applications such as immunoprecipitation, immunofluorescence, live cell imaging, biosensor assays, and protein purification.
Chromotek is now part of Proteintech
All ChromoTek Nanobody-based reagents are now available on ptglab.com.
Nano-Traps
Nano-Traps are ideal for fast, reliable and efficient one-step immunoprecipitation and co-immunoprecipitation. Nano-Traps comprise of Nanobodies conjugated to beads.
Nano-Secondary reagents
Nano-Secondary® reagents use a novel class of secondary antibodies for higher resolution & cleaner images. These reagents consist of Nanobodies conjugated to fluorescent dyes binding to conventional antibodies.
Chromobodies
Chromobodies® are fluorescent nanoprobes for real-time and live-cell imaging of intracellular endogenous proteins. Chromobodies are Nanobodies that are genetically fused to a fluorescent protein such as GFP.
Primary VHHs
Nano-Primary are highly specific, recombinantly produced alpaca single domain VHHs (Nanobodies) against a range of protein-tags, various intra- and –extracellular targets, and Immuno-Oncology targets. Their small size and high affinity make them ideal tools for applications like Imaging and Flow-Cytometry.
Nano-Caps
Nano-Caps® are optimized for effective and cost-efficient protein purification. Nano-Caps consist of Nanobodies conjugated to agarose.
Nano-CaptureLigands
Nano-CaptureLigands are optimized to specifically immobilize human, mouse, and rabbit antibodies to streptavidin/avidin. Nano-CaptureLigands are biotinylated Nanobodies.
Spot-Tag System
Proteintech's proprietary peptide-tag system for immunoprecipitation, immunofluorescence, and protein purification. Depending on application, Spot Nanobodies are conjugated to agarose, beads, or fluorescent dyes.
Immunoprecipitation
Nanobody based reagents show less background, i.e., no heavy and light antibody chains in SDS/PAGE and Western blot.
Immunofluorescence
Due to the small size of Nanobodies, higher image resolution can be achieved.
Live Cell Imaging
Chromobodies® visualize endogenous cellular structures and processes in live cells and real-time.
Western Blotting
Fluorescently labeled Nanobodies and antibodies allow for multiplexing.
Super-resolution Microscopy
The small size of Nanobodies enables better tissue penetration and decreases the distance between epitope and label.
Mass Spectrometry
Nanobody based tools enable for high reproducibility and low background.
Protein Purification
Affinity resins with Nanobodies allow for effective one-step protein purification.
Spot Capture and Detection System
Universal capture and detection system based on Spot-Nanobody for immunofluorescence, immunoprecipitation, protein purification, Western blotting, etc.
On-bead Enzyme Assays
The enzymatic activity can be assessed directly after immunoprecipitation from a cell lysate with Nanobodies.
High Content Analysis
Using Chromobodies, dynamic cellular changes can be visualized and monitored in real time.
Ligand Capture and Affinity Determination
Directed immobilization of antibodies for characterization of biomolecular interactions such as surface plasmon resonance (SPR) or bio-layer interferometry (BLI).
ELISA
An Enzyme-linked immunosorbent assay (ELISA) is method for the detection of a target molecule in a liquid sample.
Products
GFP-Trap® Agarose
An affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.
| Cat No: | Publications |
| gta | 2,863 |
V5-Trap® Magnetic Agarose
An affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/ VHH coupled to magnetic agarose beads
| Cat No: | Applications |
| v5tma | IP, CoIP, ChIP, RIP |
Myc-Trap® Agarose
An affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.
| Cat No: | Publications |
| yta | 77 |
Alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 647
Consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.
| Cat No: | Applications |
| sms1AF647-1 | IF, WB, FC |
Spot-Label ATTO594
Anti-Spot-Tag VHH/ Nanobody conjugated to ATTO594.
| Cat No: | Applications |
| eba594 | IF, WB |
Actin-Chromobody® plasmid (TagRFP)
DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.
| Cat No: | Publications |
| acr | 7 |
RFP-Trap® Magnetic Particles M-270
RFP VHH/ Nanobody coupled to Magnetic Particles M-270.
| Cat No: | Applications |
| rtd | IP, CoIP |
Articles
About Nanobodies
Discovery of Nanobodies, the Alpaca Nanobody advantage and validation of Nanobodies.
Non visible textSpot Capture & Detection System
Universal capture and detection system based on Spot-Tag®, an inert 12 amino acid peptide-tag and Spot-Nanobody for immunofluorescence, immunoprecipitation, protein purification, Western blotting, etc.
Non visible textFree Samples
Request a free sample and test the GFP-Trap & other Nano-Traps yourself.
Non visible textAbout Nanobodies
Discovery of Nanobodies, the Alpaca Nanobody advantage and validation of Nanobodies.