Mouse cGAS Recombinant antibody, PBS Only

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  WB analysis using 84045-1-RR (same clone as 84045-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84045-1-RR (cGAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 84045-1-RR (same clone as 84045-1-PBS)

  IP result of anti-cGAS (IP:84045-1-RR, 4ug; Detection:84045-1-RR 1:600) with NIH/3T3 cells lysate 1920 ug. This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 84045-1-RR (same clone as 84045-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using cGAS antibody (84045-1-RR, Clone: 240964E2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84045-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84045-1-RR against Mouse cGAS were performed. The affinity constant is below 1 pM.

cGAS Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Cat No. 84045-1-PBS

Clone No.240964E2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, IP, ELISA

Synonyms

240964E2, m cGAS, Mb21d1, mcGAS, m-cGAS

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Product Information

84045-1-PBS targets cGAS in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: cGAS fusion protein Ag31677
• Full Name: RIKEN cDNA E330016A19 gene
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC145651
• Gene Symbol: cGAS
• Gene ID (NCBI): 214763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8C6L5
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.