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cGAS Recombinant antibody

cGAS Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240964E2

Cat no : 84045-1-RR

Synonyms

m-cGAS, mcGAS, Mb21d1, m cGAS, 240964E2

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using cGAS Recombinant antibody (84045-1-RR)

    WB analysis of RAW 264.7 using 84045-1-RR

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 84045-1-RR (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using cGAS Recombinant antibody (84045-1-RR)

    IF Staining of RAW 264.7 using 84045-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using cGAS antibody (84045-1-RR, Clone: 240964E2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84045-1-RR

    Affinity and Kinetic Characterization of 84045-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84045-1-RR against Mouse cGAS were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inRAW 264.7 cells
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84045-1-RR is cGAS Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen cGAS fusion protein Ag31677
Full Name RIKEN cDNA E330016A19 gene
Calculated Molecular Weight58 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberBC145651
Gene Symbol cGAS
Gene ID (NCBI) 214763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.

Protocols

Product Specific Protocols
WB protocol for cGAS antibody 84045-1-RRDownload protocol
IF protocol for cGAS antibody 84045-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols