ChromoTek Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 647 [CTK0101, CTK0102]
Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.
Applications
IF, WB, FC
Conjugate
Alexa Fluor® 647
Host
Alpaca
Reactivity
Rabbit, Human, Macaque
Cat no : srbAF647-1
Synonyms
Validation Data Gallery
Product Information
Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.
| Description | Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH reagent uses a novel class of anti-rabbit IgG and anti-human IgG specific antibodies. This secondary antibody product consists of Nanobodies that bind to rabbit IgG and human IgG with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below. |
| Applications | IF, WB, FC |
| Host | Alpaca |
| Species Reactivity | Rabbit, Human, Macaque No cross-reactivity to mouse, rat, sheep, goat, and guinea pig IgG |
| Conjugate | Alexa Fluor® 647 |
| Physical State | Liquid |
| Suggested Dilution | Immunofluorescence 1:1,000 Super-resolution microscopy 1:1,000 Western blot 1:1,000 |
| Purification Method | Recombinant expression, affinity purification IMAC |
| Type | Mixture of 2 monoclonal Nanobodies |
| Class | Recombinant |
| Affinity (KD) | CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
| Storage Condition | Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. |
| RRID | AB_2827587 |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
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| SDS_Nano-Secondary® alpaca anti-human IgG, anti-rabbit IgG, Alexa Fluor® 647 |
| Protocol |
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| Protocol Nano-Secondary® alpaca anti-human IgG, anti-rabbit IgG, Alexa Fluor® 647 |
| Application note |
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| One-step incubation with Nano-Secondaries (PDF) |
| Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
|---|---|
Biosens Bioelectron 3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing | |
Vet Microbiol NDV inhibited IFN-β secretion through impeding CHCHD10-mediated mitochondrial fusion to promote viral proliferation | |
Nat Methods Spatial single-cell mass spectrometry defines zonation of the hepatocyte proteome |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Clarisse (Verified Customer) (08-08-2022) | This secondary nano-body performs incredibly well in both single-step and sequential IF incubations. The dilution used was 1:1000. The signal is way more specific compared to conventional alexa secondaries. You should definitely give it a try!
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FH Joe (Verified Customer) (04-26-2022) | I used this secondary in an indirect flow cytometry experiment, staining for a phospho-protein. I found that this secondary resulted in a stronger signal when compared to my regular life scientific alexa fluor secondary antibody. I will be using these nanobodies from now on! The antibody was used at a dilution of 1:4000, 30m RT staining.
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