ChromoTek Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 568 [CTK0101, CTK0102]

Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.

Applications

IF, WB, FC

Conjugate

Alexa Fluor® 568

Host

Alpaca

Reactivity

Rabbit, Human, Macaque

Cat no : srbAF568-1

Synonyms

human IgG, rabbit IgG, immunoglobulin Gamma



Product Information

Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.

DescriptionNano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH reagent uses a novel class of anti-rabbit IgG and anti-human IgG specific antibodies. This secondary antibody product consists of Nanobodies that bind to rabbit IgG and human IgG with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
ApplicationsIF, WB, FC
HostAlpaca
Species ReactivityRabbit, Human, Macaque
No cross-reactivity to mouse, rat, sheep, goat, and guinea pig IgG
ConjugateAlexa Fluor® 568
Physical StateLiquid
Suggested DilutionImmunofluorescence 1:1,000
Super-resolution microscopy 1:1,000
Western blot 1:1,000
Purification MethodRecombinant expression, affinity purification IMAC
TypeMixture of 2 monoclonal Nanobodies
ClassRecombinant
Affinity (KD) CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionStore at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.
RRIDAB_2827586
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Sci Adv

Mutation of SLC7A14 causes auditory neuropathy and retinitis pigmentosa mediated by lysosomal dysfunction.

Authors - Kimberlee P Giffen
IF

Gene Ther

A new protocol for whole-brain biodistribution analysis of AAVs by tissue clearing, light-sheet microscopy and semi-automated spatial quantification

Authors - Miguel M Lopes
IF

Neuroscience

Translocator Protein Regulate Polarization Phenotype Transformation of Microglia after Cerebral Ischemia-reperfusion Injury

Authors - Shadamu Yusuying
IF

Front Bioeng Biotechnol

Maintenance of multipotency of bone marrow mesenchymal stem cells on poly(ε-caprolactone) nanoneedle arrays through the enhancement of cell-cell interaction

Authors - Xiaoxue Ren
IF

Phytomedicine

Hesperidin inhibits lung fibroblast senescence via IL-6/STAT3 signaling pathway to suppress pulmonary fibrosis

Authors - Di Han
IF

EMBO Rep

Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome

Authors - Fakun Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Davide (Verified Customer) (06-13-2023)

The secondary antibody worked very well and the spatial resolution was great on 3d human primary cell cultures. On the image the yellow is the antibody used. The primary antibody was a ck19 rabbit.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cholangiocyte organoids