CoraLite®594-conjugated ZP3 Polyclonal antibody

ZP3 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-21279

Synonyms

Sperm receptor, Zona pellucida glycoprotein 3, Zona pellucida protein C, Zp 3, ZP3, ZP3A, ZP3A/ZP3B, ZP3B, ZPC

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Validation Data Gallery

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  IF Staining of mouse ovary using CL594-21279

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using CoraLite®594 ZP3 antibody (CL594-21279) at dilution of 1:200.

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  IF Staining of mouse ovary using CL594-21279

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using CoraLite®594 ZP3 antibody (CL594-21279) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse ovary tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-21279 is ZP3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZP3 fusion protein Ag15791
• Full Name: zona pellucida glycoprotein 3 (sperm receptor)
• Calculated Molecular Weight: 373 aa, 41 kDa
• Observed Molecular Weight: 47 kDa, 60 kDa
• GenBank Accession Number: BC113949
• Gene Symbol: ZP3
• Gene ID (NCBI): 7784
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that are processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).