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ZO1 Recombinant antibody, PBS Only

ZO1 Recombinant Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240150D7

Cat no : 82870-7-PBS

Synonyms

DKFZp686M05161, Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, Zona occludens protein 1, Zonula occludens protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZO-1 Recombinant antibody (82870-7-RR)

    WB analysis using 82870-7-RR (same clone as 82870-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82870-7-RR (ZO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZO-1 Recombinant antibody (82870-7-RR)

    WB analysis using 82870-7-RR (same clone as 82870-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82870-7-RR (ZO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ZO-1 Recombinant antibody (82870-7-RR)

    IP experiment of HEK-293 using 82870-7-RR (same clone as 82870-7-PBS)

    IP result of anti-ZO-1 (IP:82870-7-RR, 4ug; Detection:82870-7-RR 1:4000) with HEK-293 cells lysate 1610 ug. This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ZO-1 Recombinant antibody (82870-7-RR)

    IF Staining of MCF-7 using 82870-7-RR (same clone as 82870-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 82870-7-PBS is ZO1 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZO1 fusion protein Ag33182
Full Name tight junction protein 1 (zona occludens 1)
Calculated Molecular Weight 1748 aa, 195 kDa
GenBank Accession NumberBC111712
Gene Symbol ZO-1
Gene ID (NCBI) 7082
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.