ZO-1 Recombinant antibody, PBS Only

ZO-1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

240150D7

Cat no : 82870-7-PBS

Synonyms

TJP1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1, Tight junction protein 1

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Validation Data Gallery

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  WB analysis using 82870-7-RR (same clone as 82870-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82870-7-RR (ZO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

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  WB analysis using 82870-7-RR (same clone as 82870-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82870-7-RR (ZO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 82870-7-RR (same clone as 82870-7-PBS)

  IP result of anti-ZO-1 (IP:82870-7-RR, 4ug; Detection:82870-7-RR 1:4000) with HEK-293 cells lysate 1610 ug. This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82870-7-RR (same clone as 82870-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82870-7-RR (ZO-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 82870-7-RR (same clone as 82870-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82870-7-RR (same clone as 82870-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82870-7-RR (same clone as 82870-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 82870-7-RR (same clone as 82870-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 82870-7-RR (same clone as 82870-7-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZO-1 Recombinant antibody (82870-7-RR, Clone:240150D7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82870-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82870-7-RR against Human ZO-1 were performed. The affinity constant is 16.2 nM.

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Product Information

The immunogen of 82870-7-PBS is ZO-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZO-1 fusion protein Ag33182
• Full Name: tight junction protein 1 (zona occludens 1)
• Calculated Molecular Weight: 1748 aa, 195 kDa
• Observed Molecular Weight: 230 kDa
• GenBank Accession Number: BC111712
• Gene Symbol: ZO-1
• Gene ID (NCBI): 7082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).