CoraLite® Plus 750-conjugated ZO-2 Recombinant antibody

• Featured Product
• KD/KO Validated

ZO-2 Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230474D1

Cat no : CL750-83111

Synonyms

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

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Validation Data Gallery

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  WB analysis using CL750-83111

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83111 (ZO-2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using CL750-83111

  1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated ZO-2 Recombinant antibody (CL750-83111, Clone:230474D1)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-83111 targets ZO-2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZO-2 fusion protein Ag13534
• Full Name: tight junction protein 2 (zona occludens 2)
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: ~150 kDa
• GenBank Accession Number: BC027592
• Gene Symbol: TJP2
• Gene ID (NCBI): 9414
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.