ZO-2 Recombinant antibody

ZO-2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230474H8

Cat no : 83111-4-RR

Synonyms

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2



Tested Applications

Positive WB detected inHEK-293 cells, C6 cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83111-4-RR is ZO-2 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZO-2 fusion protein Ag13534
Full Name tight junction protein 2 (zona occludens 2)
Calculated Molecular Weight 134 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC027592
Gene Symbol TJP2
Gene ID (NCBI) 9414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.

Protocols

Product Specific Protocols
WB protocol for ZO-2 antibody 83111-4-RRDownload protocol
IF protocol for ZO-2 antibody 83111-4-RRDownload protocol
FC protocol for ZO-2 antibody 83111-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols