ZO-2 Recombinant antibody

ZO-2 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

230474H8

Cat no : 83111-4-RR

Synonyms

Tight junction protein 2, Tight junction protein ZO 2, TJP2, X104, ZO 2, ZO2, Zona occludens protein 2, Zonula occludens protein 2

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Validation Data Gallery

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  WB analysis using 83111-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83111-4-RR (ZO-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 83111-4-RR

  C6 cells were subjected to SDS PAGE followed by western blot with 83111-4-RR (ZO-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 83111-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-2 antibody (83111-4-RR, Clone: 230474H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity experiments of 83111-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83111-4-RR against Human ZO-2 were performed. The affinity constant is 0.776 nM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, C6 cells, HepG2 cells, MCF-7 cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83111-4-RR is ZO-2 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZO-2 fusion protein Ag13534
• Full Name: tight junction protein 2 (zona occludens 2)
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC027592
• Gene Symbol: TJP2
• Gene ID (NCBI): 9414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.