ZO-2 Recombinant antibody
ZO-2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
230474H8
Cat no : 83111-4-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, C6 cells, HepG2 cells, MCF-7 cells |
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | A431 cells, U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83111-4-RR is ZO-2 Fusion Protein expressed in E. coli.
Tested Reactivity | human, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | ZO-2 fusion protein Ag13534 |
Full Name | tight junction protein 2 (zona occludens 2) |
Calculated Molecular Weight | 134 kDa |
Observed Molecular Weight | 150 kDa |
GenBank Accession Number | BC027592 |
Gene Symbol | TJP2 |
Gene ID (NCBI) | 9414 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.
Protocols
Product Specific Protocols | |
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WB protocol for ZO-2 antibody 83111-4-RR | Download protocol |
IF protocol for ZO-2 antibody 83111-4-RR | Download protocol |
FC protocol for ZO-2 antibody 83111-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |