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TJP2 Recombinant antibody, PBS Only

TJP2 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230474H8

Cat no : 83111-4-PBS

Synonyms

Tight junction protein 2, Tight junction protein ZO 2, TJP2, X104, ZO 2, ZO2, Zona occludens protein 2, Zonula occludens protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZO-2 Recombinant antibody (83111-4-RR)

    WB analysis using 83111-4-RR (same clone as 83111-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83111-4-RR (ZO-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83111-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using ZO-2 Recombinant antibody (83111-4-RR)

    WB analysis of C6 using 83111-4-RR (same clone as 83111-4-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 83111-4-RR (ZO-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83111-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ZO-2 Recombinant antibody (83111-4-RR)

    IF Staining of MCF-7 using 83111-4-RR (same clone as 83111-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-2 antibody (83111-4-RR, Clone: 230474H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83111-4-PBS in a different storage buffer formulation.

  • Affinity experiments of 83111-4-RR

    Affinity experiments of 83111-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83111-4-RR against Human ZO-2 were performed. The affinity constant is 0.776 nM.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83111-4-PBS is TJP2 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TJP2 fusion protein Ag13534
Full Name tight junction protein 2 (zona occludens 2)
Calculated Molecular Weight 134 kDa
GenBank Accession NumberBC027592
Gene Symbol TJP2
Gene ID (NCBI) 9414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.