ZO-2 Recombinant antibody, PBS Only

ZO-2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230474H8

Cat no : 83111-4-PBS

Synonyms

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

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Validation Data Gallery

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  WB analysis using 83111-4-RR (same clone as 83111-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83111-4-RR (ZO-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83111-4-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 83111-4-RR (same clone as 83111-4-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 83111-4-RR (ZO-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83111-4-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 83111-4-RR (same clone as 83111-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-2 antibody (83111-4-RR, Clone: 230474H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83111-4-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83111-4-RR (same clone as 83111-4-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug ZO-2 Recombinant antibody (83111-4-RR, Clone:230474H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83111-4-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 83111-4-RR (same clone as 83111-4-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug ZO-2 Recombinant antibody (83111-4-RR, Clone:230474H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83111-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83111-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83111-4-RR against Human XRN2 were performed. The affinity constant is 80.7 pM.

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Product Information

The immunogen of 83111-4-PBS is ZO-2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZO-2 fusion protein Ag13534
• Full Name: tight junction protein 2 (zona occludens 2)
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC027592
• Gene Symbol: TJP2
• Gene ID (NCBI): 9414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.