CoraLite®594-conjugated ZO-1 Recombinant antibody

ZO-1 Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

240150D7

Cat no : CL594-82870-7

Synonyms

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Validation Data Gallery

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  IF Staining of MCF-7 using CL594-82870-7

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 ZO-1 antibody (CL594-82870-7, Clone: 240150D7 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Freshly prepared samples are recommended.

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-82870-7 is ZO-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZO-1 fusion protein Ag33182
• Full Name: tight junction protein 1 (zona occludens 1)
• Calculated Molecular Weight: 1748 aa, 195 kDa
• Observed Molecular Weight: 230 kDa
• GenBank Accession Number: BC111712
• Gene Symbol: ZO-1
• Gene ID (NCBI): 7082
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).