ZNF217 Recombinant antibody

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  WB analysis using 84276-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84276-1-RR (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 84276-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 84276-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 84276-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84276-1-RR against Human ZNF217 were performed. The affinity constant is below 1 pM.

ZNF217 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84276-1-RR

Clone No.241217E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ZFP217, Zinc finger protein 217, ZABC1, 241217E3

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Tested Applications

• Positive WB detected in: HepG2 cells, LNCaP cells, Caco-2 cells, PC-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84276-1-RR targets ZNF217 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZNF217 fusion protein Ag19453
• Full Name: zinc finger protein 217
• Calculated Molecular Weight: 1048 aa, 115 kDa
• Observed Molecular Weight: ~ 130 kDa
• GenBank Accession Number: BC113427
• Gene Symbol: ZNF217
• Gene ID (NCBI): 7764
• RRID: AB_3671822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O75362
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger protein 217 (ZNF217) is a member of the Krüppel-like factor transcription factor family. ZNF217 possesses a characteristic structure of zinc finger motifs and plays a crucial role in regulating the biological activities of cells. ZNF217 binds to DNA through its zinc fingers thereby targeting potential protein binding partners to specific sites in the genome. Recent findings have revealed that ZNF217 is strongly associated with multiple aspects of cancer progression, impacting patient prognosis. PMID: 38259608