ZMPSTE24 Recombinant antibody

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  WB analysis using 84547-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84547-1-RR (ZMPSTE24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84547-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84547-1-RR against Human ZMPSTE24 were performed. The affinity constant is 1.87 nM.

ZMPSTE24 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84547-1-RR

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FACE-1, FACE1, FACE 1, EC:3.4.24.84, CAAX prenyl protease 1 homolog

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, PC-3 cells, SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84547-1-RR targets ZMPSTE24 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZMPSTE24 fusion protein Ag33613
• Full Name: zinc metallopeptidase (STE24 homolog, S. cerevisiae)
• Calculated Molecular Weight: 475 aa, 55 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC037283
• Gene Symbol: ZMPSTE24
• Gene ID (NCBI): 10269
• RRID: AB_3672053
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O75844
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZMPSTE24(CAAX prenyl protease 1 homolog) gene encodes a zinc metalloproteinase involved in the processing of farnesylated proteins(PubMed: 10373325). The significance of ZMPSTE24 in human disease stems from its role as an enzyme necessary for the correct processing and maturation of lamin A ,an intermediate filament component of the nuclear envelope (PubMed: 16297189).