ZFYVE26 Polyclonal antibody

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  IHC staining of mouse heart using 55418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55418-1-AP (ZFYVE26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 55418-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ZFYVE26 antibody (55418-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ZFYVE26 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 55418-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

KIAA0321, Spastizin, SPG15, ZFYVE26

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Tested Applications

• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55418-1-AP targets ZFYVE26 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: zinc finger, FYVE domain containing 26
• Calculated Molecular Weight: 285 kDa
• GenBank Accession Number: NM_015346
• Gene Symbol: ZFYVE26
• Gene ID (NCBI): 23503
• RRID: AB_3670251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q68DK2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZFYVE26 is a large protein of 2539 amino acid residues and contains a FYVE domain that confers to the protein binding affinity to the membrane-associated phosphatidylinositol 3-phosphate (PtdIns3P). ZFYVE26 has a diffuse cytoplasmic distribution and partially colocalizes with early endosomes, the endoplasmic reticulum, microtubules, and vesicles. It localizes also to the midbody during cytokinesis (PMID: 24284334).