Tested Applications
Positive IF/ICC detected in | A549 cells |
Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83909-5-RR targets ZFP161 / ZBTB14 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | ZFP161 / ZBTB14 fusion protein Ag16125 Predict reactive species |
Full Name | zinc finger protein 161 homolog (mouse) |
Calculated Molecular Weight | 449 aa, 51 kDa |
GenBank Accession Number | BC110519 |
Gene Symbol | ZFP161 |
Gene ID (NCBI) | 7541 |
RRID | AB_3671491 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | O43829 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene.
Protocols
Product Specific Protocols | |
---|---|
IF protocol for ZFP161 / ZBTB14 antibody 83909-5-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |