ZEB2 Recombinant antibody

ZEB2 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1K22

Cat no : 82020-1-RR

Synonyms

FLJ42816, KIAA0569, SIP 1, SIP1, Smad interacting protein 1, SMADIP1, ZEB2, ZFHX1B, ZFX1B

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Validation Data Gallery

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  WB analysis using 82020-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82020-1-RR (ZEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 82020-1-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82020-1-RR (ZEB2 antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 82020-1-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82020-1-RR (ZEB2 antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 82020-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ZEB2 antibody (82020-1-RR, Clone: 1K22 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 82020-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ZEB2 antibody (82020-1-RR, Clone: 1K22 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, A549 cells, K-562 cells
• Positive IHC detected in: human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82020-1-RR targets ZEB2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZEB2 fusion protein Ag5171
• Full Name: zinc finger E-box binding homeobox 2
• Calculated Molecular Weight: 136 kDa
• Observed Molecular Weight: 140-150 kD
• GenBank Accession Number: BC060819
• Gene Symbol: ZEB2
• Gene ID (NCBI): 9839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. PMID: 27659015 )