ZCCHC2 Polyclonal antibody

ZCCHC2 Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Conjugate

Unconjugated

Cat no : 25741-1-AP

Synonyms

KIAA1744, C18orf49

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Validation Data Gallery

• 

  IP experiment of HeLa using 25741-1-AP

  IP result of anti-ZCCHC2 (IP:25741-1-AP, 4ug; Detection:25741-1-AP 1:500) with HeLa cells lysate 1800 ug.

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Tested Applications

• Positive IP detected in: HeLa cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25741-1-AP targets ZCCHC2 in IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZCCHC2 fusion protein Ag22744
• Full Name: zinc finger, CCHC domain containing 2
• Calculated Molecular Weight: 1178 aa, 126 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC006340
• Gene Symbol: ZCCHC2
• Gene ID (NCBI): 54877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZCCHC2 is a poorly characterized protein of 126 kDa with long intrinsically disordered regions, a PX domain, and a CCHC-type zinc-finger (ZnF) domain. ZCCHC2 is distantly related to ZCCHC14 but lacks the SAM domain that is known to interact with the CNGGN pentaloop in 1E and PRE. ZCCHC2 is a critical host factor for K5. ZCCHC2 recruits the terminal nucleotidyl transferase TENT4 to elongate poly(A) tails with mixed sequences, delaying deadenylation. The observed molecular weight is consistent with what has been described in the literature (PMID: 37413987).