ZBP1 Recombinant antibody

ZBP1 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241582E2

Cat no : 84396-2-RR

Synonyms

Tumor stroma and activated macrophage protein DLM-1, DLM1, DLM 1, DAI, C20orf183

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Validation Data Gallery

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  IHC staining of human colon cancer using 84396-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84396-2-RR (ZBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84396-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBP1 antibody (84396-2-RR, Clone: 241582E2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84396-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84396-2-RR against Human ZBP1 were performed. The affinity constant is 5.12 nM.

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Tested Applications

• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84396-2-RR targets ZBP1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZBP1 fusion protein Ag4119
• Full Name: Z-DNA binding protein 1
• Calculated Molecular Weight: 46 kDa
• GenBank Accession Number: BC028218
• Gene Symbol: ZBP1
• Gene ID (NCBI): 81030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).