ZBP1 Recombinant antibody, PBS Only (Detector)

ZBP1 Uni-rAb^TM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241582E2

Cat no : 84396-2-PBS

Synonyms

Tumor stroma and activated macrophage protein DLM-1, DLM1, DLM 1, DAI, C20orf183

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01267-1

  Sandwich ELISA standard curve of MP01267-1, Human ZBP1 Recombinant Matched Antibody Pair - PBS only. 84396-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4119. 84396-2-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

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  Sample test of MP01267-1

  The mean ZBP1 concentration was determined to be 7.1 ng/mL in HeLa cell extract based on a 2.8 mg/mL extract load, 15.4 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load and 9.3 ng/mL in HL-60 cell extract based on a 1.4 mg/mL extract load.

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  IF Staining of HeLa using 84396-2-RR (same clone as 84396-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBP1 antibody (84396-2-RR, Clone: 241582E2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84396-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01267-1

  Cytometric bead array standard curve of MP01267-1, ZBP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84396-1-PBS. Detection antibody: 84396-2-PBS. Standard: Ag4119. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84396-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84396-2-RR against Human ZBP1 were performed. The affinity constant is 5.12 nM.

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Product Information

84396-2-PBS targets ZBP1 as part of a matched antibody pair:

MP01267-1: 84396-1-PBS capture and 84396-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZBP1 fusion protein Ag4119
• Full Name: Z-DNA binding protein 1
• Calculated Molecular Weight: 46 kDa
• GenBank Accession Number: BC028218
• Gene Symbol: ZBP1
• Gene ID (NCBI): 81030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).