ZAK Monoclonal antibody, PBS Only

• 

  WB analysis using 68599-1-Ig (same clone as 68599-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 68599-1-Ig (same clone as 68599-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit skeletal muscle using 68599-1-Ig (same clone as 68599-1-PBS)

  rabbit skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

ZAK Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68599-1-PBS

Clone No.2H10E4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, rabbit

Applications

WB, Indirect ELISA

Synonyms

AZK, HCCS 4, MLK related kinase, MLK7, mlklak, MLT, MLTK, MRK, ZAK

---

Product Information

68599-1-PBS targets ZAK in WB, Indirect ELISA applications and shows reactivity with Human, rat, rabbit samples.

• Tested Reactivity: Human, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZAK fusion protein Ag6747
• Full Name: sterile alpha motif and leucine zipper containing kinase AZK
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC001401
• Gene Symbol: ZAK
• Gene ID (NCBI): 51776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NYL2
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.