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ZAK Monoclonal antibody

ZAK Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H10E4

Cat no : 68599-1-Ig

Synonyms

AZK, HCCS 4, MLK related kinase, MLK7, mlklak, MLT, MLTK, MRK, ZAK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZAK Monoclonal antibody (68599-1-Ig)

    WB analysis using 68599-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using ZAK Monoclonal antibody (68599-1-Ig)

    WB analysis of rat skeletal muscle using 68599-1-Ig

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit skeletal muscle tissue using ZAK Monoclonal antibody (68599-1-Ig)

    WB analysis of rabbit skeletal muscle using 68599-1-Ig

    rabbit skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU2OS cells, rat skeletal muscle tissue, rabbit skeletal muscle tissue, Saos-2 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68599-1-Ig targets ZAK in WB, ELISA applications and shows reactivity with Human, rat, rabbit samples.

Tested Reactivity Human, rat, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ZAK fusion protein Ag6747
Full Name sterile alpha motif and leucine zipper containing kinase AZK
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 91 kDa
GenBank Accession NumberBC001401
Gene Symbol ZAK
Gene ID (NCBI) 51776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.

Protocols

Product Specific Protocols
WB protocol for ZAK antibody 68599-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols