YY2 Monoclonal antibody

YY2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1C11A7

Cat no : 66839-1-Ig

Synonyms

Transcription factor YY2, Yin and yang 2, YY 2, YY2, YY2 transcription factor, Zinc finger protein 631, ZNF631

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Validation Data Gallery

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  WB analysis of human kidney using 66839-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66839-1-Ig (YY2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 66839-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66839-1-Ig (YY2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66839-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66839-1-Ig (YY2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human kidney tissue, human placenta tissue, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66839-1-Ig targets YY2 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: YY2 fusion protein Ag25373
• Full Name: YY2 transcription factor
• Calculated Molecular Weight: 372 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC137217
• Gene Symbol: YY2
• Gene ID (NCBI): 404281
• RRID: AB_2882182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YY2 (Yin-Yang 2) encodes a zinc finger transcription factor similarity to the well-characterized YY1 that locating to nucleus. Both proteins recognize an identical DNA-binding motifs with a high homologous Zinc Finger region, and these transcription factors can act as activators or repressors when bound to DNA (PMID:26350623). It is reported that YY2 is placental mammal-specific and is absent in marsupial and non-mammalian vertebrate species (PMID:16377127). YYs are play an important role in multiple developmental processes , such as implantation, cell differentiation, X inactivation, imprinting and organogenesis (PMID:27191592). YY2 is expressed in kindey, liver, spleen and testicle but not in the colon (PMID:15087442). A 41kDa band could be detected.

Publications

Species	Application	Title
human	WB	Cell Death Dis; REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.; Authors - Yunshang Chen