Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66281 targets YY1 in IF/ICC applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | YY1 fusion protein Ag17732 Predict reactive species |
| Full Name | YY1 transcription factor |
| Calculated Molecular Weight | 414 aa, 45 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC037308 |
| Gene Symbol | YY1 |
| Gene ID (NCBI) | 7528 |
| RRID | AB_2883302 |
| Conjugate | CoraLite®488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 491 nm / 516 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25490 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL488 YY1 antibody CL488-66281 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
