YTHDC2 Recombinant antibody, PBS Only (Detector)

YTHDC2 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241123A9

Cat no : 83970-1-PBS

Synonyms

YTH domain-containing protein 2, hYTHDC2, EC:3.6.4.13, 3'-5' RNA helicase YTHDC2, 241123A9

---

Validation Data Gallery

• 

  WB analysis using 83970-1-RR (same clone as 83970-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83970-1-RR (YTHDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00893-3

  Sandwich ELISA standard curve of MP00893-3, Human YTHDC2 Recombinant Matched Antibody Pair - PBS only. 83970-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26690. 83970-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL

• 

  Sample test of MP00893-3

  The mean YTHDC2 concentration was determined to be 4.89 ng/mL in HEK-293T cell extract based on a 1.4 mg/mL extract load.

• 

  IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83970-1-RR (same clone as 83970-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug YTHDC2 Recombinant antibody (83970-1-RR, Clone:241123A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00893-1

  Cytometric bead array standard curve of MP00893-1, YTHDC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83970-4-PBS. Detection antibody: 83970-1-PBS. Standard: Ag26690. Range: 0.156-20 ng/mL.

---

Product Information

83970-1-PBS targets YTHDC2 as part of a matched antibody pair:

MP00893-1: 83970-4-PBS capture and 83970-1-PBS detection (validated in Cytometric bead array)

MP00893-3: 83970-3-PBS capture and 83970-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: YTHDC2 fusion protein Ag26690
• Full Name: YTH domain containing 2
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC137285
• Gene Symbol: YTHDC2
• Gene ID (NCBI): 64848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.