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YTHDC2 Recombinant antibody

YTHDC2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241123A9

Cat no : 83970-1-RR

Synonyms

YTH domain-containing protein 2, hYTHDC2, EC:3.6.4.13, 3'-5' RNA helicase YTHDC2, 241123A9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YTHDC2 Recombinant antibody (83970-1-RR)

    WB analysis using 83970-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83970-1-RR (YTHDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat testis tissue using YTHDC2 Recombinant antibody (83970-1-RR)

    IHC staining of rat testis using 83970-1-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using YTHDC2 Recombinant antibody (83970-1-RR)

    IHC staining of rat testis using 83970-1-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using YTHDC2 Recombinant antibody (83970-1-RR)

    FC experiment of HepG2 using 83970-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug YTHDC2 Recombinant antibody (83970-1-RR, Clone:241123A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, A549 cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83970-1-RR is YTHDC2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YTHDC2 fusion protein Ag26690
Full Name YTH domain containing 2
Observed Molecular Weight160 kDa
GenBank Accession NumberBC137285
Gene Symbol YTHDC2
Gene ID (NCBI) 64848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.

Protocols

Product Specific Protocols
WB protocol for YTHDC2 antibody 83970-1-RRDownload protocol
IHC protocol for YTHDC2 antibody 83970-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols