XRCC5 Recombinant antibody, PBS Only

XRCC5 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240301G6

Cat no : 80325-6-PBS

Synonyms

86 kDa subunit of Ku antigen, CTC85, CTCBF, DNA repair protein XRCC5, FLJ39089, G22P2, KARP 1, KARP1, KU80, Ku86, KUB2, NFIV, Nuclear factor IV, Thyroid lupus autoantigen, TLAA, XRCC5

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Validation Data Gallery

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  WB analysis using 80325-6-RR (same clone as 80325-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80325-6-RR (XRCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80325-6-RR (same clone as 80325-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC5 antibody (80325-6-RR, Clone: 240301G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 80325-6-PBS is XRCC5 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: XRCC5 fusion protein Ag9454
• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
• Calculated Molecular Weight: 732 aa, 83 kDa
• GenBank Accession Number: BC019027
• Gene Symbol: XRCC5
• Gene ID (NCBI): 7520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.