XRCC4 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

XRCC4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E5D2

Cat no : 66621-1-PBS

Synonyms

DNA repair protein XRCC4, XRCC4

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Validation Data Gallery

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  WB analysis using 66621-1-Ig (same clone as 66621-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66621-1-Ig (same clone as 66621-1-PBS)

  WB result of XRCC4 antibody (66621-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XRCC4 transfected HEK-293 cells. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66621-1-Ig (same clone as 66621-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66621-1-Ig (same clone as 66621-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66621-1-Ig (same clone as 66621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66621-1-Ig (same clone as 66621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66621-1-Ig (same clone as 66621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66621-1-Ig (same clone as 66621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66621-1-PBS targets XRCC4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: XRCC4 fusion protein Ag8807
• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 4
• Calculated Molecular Weight: 310 aa, 35 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC005259
• Gene Symbol: XRCC4
• Gene ID (NCBI): 7518
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.