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  • Featured Product
  • KD/KO Validated

XRCC4 Monoclonal antibody

XRCC4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E5D2

Cat no : 66621-1-Ig

Synonyms

DNA repair protein XRCC4, XRCC4

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis of HEK-293 using 66621-1-Ig

    WB result of XRCC4 antibody (66621-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XRCC4 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis using 66621-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis of COLO 320 using 66621-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis of MCF-7 using 66621-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human colon cancer using 66621-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human colon cancer using 66621-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human breast cancer using 66621-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human breast cancer using 66621-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, COLO 320 cells, MCF-7 cells, A431 cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66621-1-Ig targets XRCC4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen XRCC4 fusion protein Ag8807
Full Name X-ray repair complementing defective repair in Chinese hamster cells 4
Calculated Molecular Weight 310 aa, 35 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC005259
Gene Symbol XRCC4
Gene ID (NCBI) 7518
RRIDAB_2881981
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for XRCC4 antibody 66621-1-IgDownload protocol
IHC protocol for XRCC4 antibody 66621-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Immunity

Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.

Authors - Yan Wang
  • KD Validated
View Article