L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 16462-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16462-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16462-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 16462-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16462-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 16462-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16462-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of L02 using 16462-1-AP
IP result of anti-XPA (IP:16462-1-AP, 4ug; Detection:16462-1-AP 1:300) with L02 cells lysate 3000 ug.
IP result of anti-XPA (IP:16462-1-AP, 4ug; Detection:16462-1-AP 1:300) with L02 cells lysate 3000 ug.
IF Staining of HepG2 using 16462-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XPA antibody (16462-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XPA antibody (16462-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HepG2 using 16462-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XPA (16462-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XPA (16462-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
XPA, also named as DNA repair protein complementing XP-A cells, is a 272 amino acid protein, which belongs to the XPA family. XPA is expressed in various cell lines and in skin fibroblasts. XPA is involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. XPA is required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. The calculated molecular weight of XPA is 31 kDa, but modified XPA protein is about 40 kDa (PMID: 17848622).
L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 16462-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16462-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16462-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 16462-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16462-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 16462-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16462-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP Figures
IP experiment of L02 using 16462-1-AP
IP result of anti-XPA (IP:16462-1-AP, 4ug; Detection:16462-1-AP 1:300) with L02 cells lysate 3000 ug.
IF/ICC Figures
IF Staining of HepG2 using 16462-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XPA antibody (16462-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC (INTRA) Figures
FC experiment of HepG2 using 16462-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XPA (16462-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
XPA Polyclonal antibody
Catalog Number
16462-1-AP
Citations
8
Dilutions
WB : 1:200-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, zebrafish
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
