CoraLite® Plus 750-conjugated XLF Monoclonal antibody
XLF Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
1F8C7
Cat no : CL750-66552
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL750-66552 targets XLF in WB applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | XLF fusion protein Ag2496 |
Full Name | nonhomologous end-joining factor 1 |
Calculated Molecular Weight | 35 kDa |
Observed Molecular Weight | 37 kDa |
GenBank Accession Number | BC030986 |
Gene Symbol | XLF |
Gene ID (NCBI) | 79840 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 750 XLF antibody CL750-66552 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |