CoraLite® Plus 488-conjugated WWP1 Monoclonal antibody

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  FC experiment of PC-3 using CL488-67804

  1x10^6 PC-3 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Wwp1 Monoclonal Antibody (CL488-67804, Clone:2G8G11)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

WWP1 Monoclonal Antibody for FC (Intra)

Cat No. CL488-67804

Clone No.2G8G11

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Synonyms

hSDRP1, HECT-type E3 ubiquitin transferase WWP1, EC:2.3.2.26, Atrophin-1-interacting protein 5, AIP5

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Tested Applications

• Positive FC (Intra) detected in: PC-3 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67804 targets WWP1 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: WWP1 fusion protein Ag30171
• Full Name: WW domain containing E3 ubiquitin protein ligase 1
• Calculated Molecular Weight: 922 aa, 105 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC036065
• Gene Symbol: WWP1
• Gene ID (NCBI): 11059
• RRID: AB_3672963
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H0M0
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.