CoraLite® Plus 488-conjugated WNT8A Polyclonal antibody

WNT8A Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-30518

Synonyms

Protein Wnt 8a, Protein Wnt 8d, WNT8A, WNT8D

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Validation Data Gallery

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  IF Staining of HCT 116 using CL488-30518

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using CoraLite® Plus 488 WNT8A antibody (CL488-30518) at dilution of 1:200, CL594-Phalloidin (red).

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  IF Staining of HCT 116 using CL488-30518

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using CoraLite® Plus 488 WNT8A antibody (CL488-30518) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF detected in: HCT 116 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-30518 targets WNT8A in IF applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WNT8A fusion protein Ag29510
• Full Name: wingless-type MMTV integration site family, member 8A
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: NM_001300938
• Gene Symbol: WNT8A
• Gene ID (NCBI): 7478
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

WNT8A,also named as WNT8D, belongs to the Wnt family of secreted signaling proteins. It is a ligand for members of the frizzled family of seven transmembrane receptors. These have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT8A may play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.