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CoraLite® Plus 488-conjugated WNT10B Monoclonal antibody

WNT10B Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G11E4

Cat no : CL488-67210

Synonyms

Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10B, WNT12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated WNT10B Monoclonal an (CL488-67210)

    IF Staining of HeLa using CL488-67210

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 WNT10B antibody (CL488-67210, Clone: 1G11E4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated WNT10B Monoclonal an (CL488-67210)

    FC experiment of HeLa using CL488-67210

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human WNT10B (CL488-67210, Clone:1G11E4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67210 targets WNT10B in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WNT10B fusion protein Ag17114
Full Name wingless-type MMTV integration site family, member 10B
Calculated Molecular Weight 389 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC096353
Gene Symbol WNT10B
Gene ID (NCBI) 7480
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 WNT10B antibody CL488-67210Download protocol
FC protocol for CL Plus 488 WNT10B antibody CL488-67210Download protocol
Standard Protocols
Click here to view our Standard Protocols