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  • KD/KO Validated

WNT10B Monoclonal antibody

WNT10B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G11E4

Cat no : 67210-1-Ig

Synonyms

Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10, WNT10B, WNT12



Tested Applications

Positive WB detected inNCCIT cells, MCF-7 cells, NCI-H1299 cells, human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inmouse liver tissue, mouse skeletal muscle tissue, human heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67210-1-Ig targets WNT10B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WNT10B fusion protein Ag17114
Full Name wingless-type MMTV integration site family, member 10B
Calculated Molecular Weight 389 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC096353
Gene Symbol WNT10B
Gene ID (NCBI) 7480
RRIDAB_2882503
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.

Protocols

Product Specific Protocols
WB protocol for WNT10B antibody 67210-1-IgDownload protocol
IHC protocol for WNT10B antibody 67210-1-IgDownload protocol
IF protocol for WNT10B antibody 67210-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Pharmacol Res

Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B.

Authors - Xiaomei Li
  • KD Validated
WB

Int J Biol Macromol

Xylooligosaccharides alleviate the carbohydrate-enriched diet-induced intestinal barrier dysfunction in carp Megalobrama amblycephala by promoting intestinal development, immunity and gut microbiota

Authors - Wei-Liang Chen
humanWB

Heliyon

MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling

Authors - Qingqing Chen