NSD3 Recombinant antibody

NSD3 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230499C2

Cat no : 83116-2-RR

Synonyms

WHSC1L1, Protein whistle, pp14328, Nuclear SET domain-containing protein 3, Histone-lysine N-methyltransferase NSD3

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Validation Data Gallery

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  WB analysis using 83116-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83116-2-RR (WHSC1L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung squamous cell carcinoma using 83116-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83116-2-RR (NSD3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 83116-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug NSD3 Recombinant antibody (83116-2-RR, Clone:230499C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83116-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83116-2-RR against Human NSD3 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells, K-562 cells
• Positive IHC detected in: human lung squamous cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83116-2-RR targets NSD3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NSD3 fusion protein Ag1894
• Full Name: Wolf-Hirschhorn syndrome candidate 1-like 1
• Calculated Molecular Weight: 1437 aa, 162 kDa
• Observed Molecular Weight: 160-170 kDa, 68-72 kDa
• GenBank Accession Number: BC012059
• Gene Symbol: WHSC1L1
• Gene ID (NCBI): 54904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).