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WEE1 Polyclonal antibody, PBS Only

WEE1 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29474-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WEE1 Polyclonal antibody (29474-1-AP)

    WB analysis using 29474-1-AP (same clone as 29474-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29474-1-AP (WEE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29474-1-PBS in a different storage buffer formulation.

Product Information

29474-1-PBS targets WEE1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WEE1 fusion protein Ag30194
Full Name WEE1 homolog (S. pombe)
Calculated Molecular Weight 72 kDa
Observed Molecular Weight72 kDa,110 kDa
GenBank Accession NumberBC051831
Gene Symbol WEE1
Gene ID (NCBI) 7465
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

WEE1 kinase is a serine-threonine kinase that regulates G2/M checkpoint transition. WEE1 triggers G2/M arrest through inhibitory phosphorylation on Tyr15 of CDK1 (Cdc2) and preventing entry into mitosis to allow DNA repair during DNA damage (PMID: 27427153).The predicted native molecular weight for Wee1 based on the intact human Wee1 cDNA sequence is 72 kDa, whereas the 95-110 kDa protein is a highly modified product (PMID: 7568188). Others have shown that Wee1 is 70-72 kDa and 49-55 kDa (PMID: 12214061).