WEE1 Polyclonal antibody

WEE1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 29474-1-AP

Synonyms

DKFZp686I18166, FLJ16446, WEE1, WEE1 homolog (S. pombe), Wee1 like protein kinase, WEE1A, Wee1A kinase, WEE1hu

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Validation Data Gallery

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  WB analysis using 29474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29474-1-AP (WEE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells, nouse brain tissue, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

29474-1-AP targets WEE1 in WB, IF, CoIP, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WEE1 fusion protein Ag30194
• Full Name: WEE1 homolog (S. pombe)
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72 kDa,110 kDa
• GenBank Accession Number: BC051831
• Gene Symbol: WEE1
• Gene ID (NCBI): 7465
• RRID: AB_2918314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WEE1 kinase is a serine-threonine kinase that regulates G2/M checkpoint transition. WEE1 triggers G2/M arrest through inhibitory phosphorylation on Tyr15 of CDK1 (Cdc2) and preventing entry into mitosis to allow DNA repair during DNA damage (PMID: 27427153).The predicted native molecular weight for Wee1 based on the intact human Wee1 cDNA sequence is 72 kDa, whereas the 95-110 kDa protein is a highly modified product (PMID: 7568188). Others have shown that Wee1 is 70-72 kDa and 49-55 kDa (PMID: 12214061).

Publications

Species	Application	Title
mouse	IF	Nucleic Acids Res; DIS3L2 ribonuclease degrades terminal-uridylated RNA to ensure oocyte maturation and female fertility; Authors - Di Wu
human	WB	J Cell Mol Med; Venetoclax enhances DNA damage induced by XPO1 inhibitors: A novel mechanism underlying the synergistic antileukaemic effect in acute myeloid leukaemia.; Authors - Hanxi Yu
mouse	WB	Reprod Toxicol; High-dose zearalenone exposure disturbs G2/M transition during mouse oocyte maturation.; Authors - Yi-Ming Ji
human	WB	Anal Cell Pathol (Amst); miR-138-5p Inhibits the Growth and Invasion of Glioma Cells by Regulating WEE1.; Authors - Jianwu Gong
human,mouse	WB,CoIP	J Pharm Pharmacol; GL-V9 synergizes with oxaliplatin of colorectal cancer via Wee1 degradation mediated by HSP90 inhibition; Authors - Hongyu Chen
mouse	WB	Ren Fail; Hyperactivation of p53 contributes to mitotic catastrophe in podocytes through regulation of the Wee1/CDK1/cyclin B1 axis; Authors - Jie Feng