WDR61 Polyclonal antibody

WDR61 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 22536-1-AP

Synonyms

REC14, SKI8, WD repeat domain 61, WDR61

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Validation Data Gallery

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  WB analysis using 22536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22536-1-AP (WDR61 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22536-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22536-1-AP (WDR61 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 22536-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22536-1-AP (WDR61 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22536-1-AP

  IP result of anti-WDR61 (IP:22536-1-AP, 4ug; Detection:22536-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse testis using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22536-1-AP (WDR61 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22536-1-AP (WDR61 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22536-1-AP (WDR61 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 22536-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22536-1-AP (WDR61 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 22536-1-AP

  Immunofluorescent analysis of HeLa cells using 22536-1-AP (WDR61 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, HepG2 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse testis tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IP: See 1 publications below

Product Information

The immunogen of 22536-1-AP is WDR61 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR61 fusion protein Ag18443
• Full Name: WD repeat domain 61
• Calculated Molecular Weight: 305 aa, 34 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC010080
• Gene Symbol: WDR61
• Gene ID (NCBI): 80349
• RRID: AB_11232419
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency (refer to UniProt). Catalog#22536-1-AP is a rabbit polyclonal antibody raised against the full-length of human WDR61. The MW of this protein is 33 kDa, and this antibody specially recognises the 33 kDa protein.

Publications

Species	Application	Title
human	WB	Sci Adv; FOXA1 O-GlcNAcylation-mediated transcriptional switch governs metastasis capacity in breast cancer; Authors - Yajie Liu
mouse	WB	Cell Death Dis; UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.; Authors - Gang Xiang
		Mol Cell; SPT6 functions in transcriptional pause/release via PAF1C recruitment; Authors - Yuki Aoi
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao