WBP2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

WBP2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B4G7

Cat no : 66585-1-PBS

Synonyms

WBP 2, WBP2, WW domain binding protein 2

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Validation Data Gallery

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  WB analysis of HEK-293 using 66585-1-Ig (same clone as 66585-1-PBS)

  WB result of WBP2 antibody (66585-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WBP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66585-1-Ig (same clone as 66585-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66585-1-Ig (WBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66585-1-Ig (same clone as 66585-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66585-1-Ig (WBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66585-1-Ig (same clone as 66585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66585-1-Ig (WBP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66585-1-Ig (same clone as 66585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66585-1-Ig (WBP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66585-1-Ig (same clone as 66585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WBP2 antibody (66585-1-Ig, Clone: 2B4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66585-1-Ig (same clone as 66585-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WBP2 antibody (66585-1-Ig, Clone: 2B4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

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Product Information

66585-1-PBS targets WBP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: WBP2 fusion protein Ag2661
• Full Name: WW domain binding protein 2
• Calculated Molecular Weight: 261 aa, 28 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC010616
• Gene Symbol: WBP2
• Gene ID (NCBI): 23558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.