TrpRS Monoclonal antibody

TrpRS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C6C3

Cat no : 67952-1-Ig

Synonyms

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

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Validation Data Gallery

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  WB analysis using 67952-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HSC-T6 using 67952-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67952-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67952-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of LNCaP using 67952-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using TrpRS antibody (67952-1-Ig, Clone: 1C6C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HSC-T6 cells, 4T1 cells, human placenta tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67952-1-Ig targets TrpRS in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TrpRS fusion protein Ag31430
• Full Name: tryptophanyl-tRNA synthetase
• Calculated Molecular Weight: 471 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC017489
• Gene Symbol: WARS1
• Gene ID (NCBI): 7453
• RRID: AB_2918704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).